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  1. Selected Literature References
  2. Frontiers | Perspectives in Super-Resolved Fluorescence Microscopy: What Comes Next? | Physics
  3. Ebook Genome Visualization By Classic Methods In Light Microscopy 2001
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Ehrenberg M. Stockholm Super-resolution microscopy at a glance. J Cell Sci. Toraldo di Francia G. Resolving power and information. J Opt Soc Am. Cremer C, Masters BR. Resolution enhancement techniques in microscopy. Eur Phys J H.

Selected Literature References

Cremer C. Optics far beyond the diffraction limit. In: Springer Handbook of Lasers and Optics. Springer PubMed Abstract. Structured illumination microscopy for superresolution. Chem Phys Chem. Blom H, Widengren J. STED microscopy—towards broadened use and scope of applications. Curr Opin Chem Biol. Single-molecule methods leap ahead.

Nat Methods 11 —8.

Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy. Science —3. Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes. Angew Chem Int Ed. High-precision structural analysis of subnuclear complexes in fixed and live cells via spatially modulated illumination SMI microscopy. Chromosome Res. Imaging intracellular fluorescent proteins at nanometer resolution. Science —5. Nat Methods 5 — SPDM: light microscopy with single-molecule resolution at the nanoscale.

Appl Phys B 93 :1— Using conventional fluorescent markers for far-field fluorescence localization nanoscopy allows resolution in the nm range. J Microsc. Extending microscopic resolution with single-molecule imaging and active control. Annu Rev Biophys. Superresolution microscopy on the basis of engineered dark states.

J Am Chem Soc. Cox S. Super-resolution imaging in live cells. Dev Biol. Subnanometre single-molecule localization, registration and distance measurements. Nature — Superresolution by localization of quantum dots using blinking statistics. Opt Express 13 — Cellular uptake of gold nanoparticles and their behavior as labels for localization microscopy. Biophys J. Studying different illumination patterns for resolution improvement in fluorescence microscopy. Opt Expr. Superresolution live imaging of plant cells using structured illumination microscopy.

Frontiers | Perspectives in Super-Resolved Fluorescence Microscopy: What Comes Next? | Physics

Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics. Science aab Superresolution imaging of biological nanostructures by spectral precision distance microscopy.

Biotechnol J. Nanosizing of fluorescent objects by spatially modulated illumination microscopy. Appl Opt. J Appl Phys. High-precision SMI microscopy size measurements by simultaneous frequency domain reconstruction of the axial point spread function. Saturated patterned excitation microscopya concept for optical resolution improvement. J Opt Soc Am A.

Ebook Genome Visualization By Classic Methods In Light Microscopy 2001

Gustafsson MGL. Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution. Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins. High-precision distance measurements and volume-conserving segmentation of objects near and below the resolution limit in three-dimensional confocal fluorescence microscopy. Principles of spectral precision distance confocal microscopy of molecular nuclear structure. Handbook of Computer Vision and Applications. Three-dimensional spectral precision distance microscopy of chromatin nanostructures after triple-colour dna labelling: a study of the bcr region on chromosome 22 and the philadelphia chromosome.

Combination of structured illumination and single molecule localization microscopy in one setup. J Opt.

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Live-cell 3D super-resolution imaging in thick biological samples. Spatially modulated illumination microscopy: online visualization of intensity distribution and prediction of nanometer precision of axial distance measurements by computer simulations. J Biomed Opt. Optimized localization analysis for single-molecule tracking and super-resolution microscopy.

Nat Meth. Visualization and resolution in localization microscopy. Spatially modulated illumination microscopy allows axial distance resolution in the nanometer range. Cryogenic localization of single molecules with angstrom precision. Hell SW, Kroug M. Ground-state-depletion fluorescence microscopy: a concept for breaking the diffraction resolution limit. Appl Phys B. Expansion microscopy. Science Single-cell hi-C reveals cell-to-cell variability in chromosome structure. Wide-field fluorescence lifetime imaging with multi-anode detectors - springer.

Methods in Molecular Biology Humana Press Fluorescence microscopy is an essential tool for imaging tagged biological structures.

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Consequentially, various methods have been developed circumventing this limit of resolution. We will survey three different concepts in connection to biological applications in radiation research without making a claim to be complete. Nature Protocols Super-resolution techniques have begun to transform biological and biomedical research by allowing researchers to observe structures well below the classic diffraction limit of light.

DNA points accumulation for imaging in nanoscale topography. In DNA-PAINT, transient binding of short dye-labeled 'imager' oligonucleotides to their complementary target 'docking' strands creates the necessary 'blinking' to enable stochastic super-resolution microscopy. Using the programmability and specificity of DNA molecules as imaging and labeling probes allows researchers to decouple blinking from dye photophysics, alleviating limitations of current super-resolution techniques, making them compatible with virtually any single-molecule-compatible dye.

This protocol describes the creation of DNA origami test samples, in situ sample preparation, multiplexed data acquisition, data simulation, super-resolution image reconstruction and post-processing such as drift correction, molecule counting qPAINT and particle averaging. Moreover, we provide an integrated software package, named Picasso, for the computational steps involved.

The protocol is designed to be modular, so that individual components can be chosen and implemented per requirements of a specific application. The procedure can be completed in 1—2 d. However, localization microscopy has been largely limited to cell cultures due to the difficulties that arise in imaging thicker tissue sections. Sample fixation and antibody staining, background fluorescence, fluorophore photoinstability, light scattering in thick sections, and sample movement create significant challenges for imaging intact tissue.

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We have developed a sample preparation and image acquisition protocol to address these challenges in rat brain slices. The sample preparation combined multiple fixation steps, saponin permeabilization, and tissue clarification.